Ocean Journal of Chemical and Petroleum Engineering (ISSN: 2771-2311) | Volume 1, Issue 1 | Research Article | Open Access

Ultrasonic Assisted Extraction of Antioxidant and Total Phenolic Content from Moringa oleifera leaves in Malaysia: Effect of process parameters

Abdul Mudalip SK*

1Department of Chemical Engineering, College of Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Kuantan, Pahang, Malaysia

2Faculty of Chemical and Process Engineering Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Kuantan, Pahang, Malaysia

3Centre for Research in Advanced Fluid and Process, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Kuantan, Pahang, Malaysia

*Correspondence to: Abdul Mudalip SK 

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Abstract

This study was conducted to investigate the antioxidant activity and total phenolic compounds of the Moringa oleifera leaves. Ultrasonic assisted extraction (UAE) technique was employed with methanol as a solvent. The temperature and sonication time were varied from 30 to 45°C and 10 to 30 min, respectively at fixed ultrasonic power of 560 Watts. A 1,1 -diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and Folin – Ciocalteu assay were used to determine the antioxidant activity and total phenolic content (TPC), respectively. The extracts obtained showed changes in the antioxidant activity and total phenolic content that with the increased of extraction temperature and sonication time. The highest antioxidant activity of 72.44 ± 0.0575 % and phenolic content of 328.87 ± 0.0516 mg (GAE)/mg) were obtained at 45℃ and 30 min, respectively. Hence, UAE is suitable to extract bioactive compounds from Moringa oleifera leaves and thus, can become an alternative for antioxidants processing method.

Keywords:

Ultrasonic intensification; Antioxidant activity; Phenolic content; Moringa oleifera; Scavenging activity

Citation:

Abdul Mudalip SK. Ultrasonic Assisted Extraction of Antioxidant and Total Phenolic Content from Moringa oleifera leaves in Malaysia: Effect of process parameters. Oce Che Pet Eng Jour.2021;1(1):1-6.