Kowloon, Hong Kong, ChinaFulltext PDF
In this study, the experimental conditions for the determination of thyroid stimulating hormone by time-resolved fluorescence immunoassay were optimized to further expand the accuracy of the detec-tion method, especially for the detection of hyperthyroidism. In this study, europium fluorescent nano-particles were linked with TSH monoclonal antibody. Different commercial detection kits, nanoparticles size, antibody dosage, bovine serum albumin, and the effects of serum on detection were studied in de-tail and optimized. We detected the fluorescence values by referring to the experimental procedures in the diagnostic kits of North Biotechnology Institute and XinBo Biotechnology Co., LTD., and determined the optimal experimental conditions according to the fluorescence values. The results show that the sensitivity of TSH detection is improved obviously after the condition optimization. This study pro-vides more accurate and reliable data for clinical detection of hyperthyroidism or further scientific research.
Time-Resolved Fluorescence Analysis; Thyroid Stimulating Hormone; Europium particle na-noprobe; Hyperthyroidism; High sensitivity detection
Liu Ying. Time-Resolved Luminescent Analysis of Thyroid Stimulating Hormone. Int Case Rep Jour. 2022;2(2):1-11.